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AlphaScreen Nickel Chelate Donor Beads, 1 mg
Alpha Donor beads conjugated to nickel chelate. This bead can be used to capture His-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
Alpha Donor beads coated with nickel chelate. These beads can be used to capture His-tagged proteins and biomolecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:
- Protein-protein interaction assays
- Protein detection assays
- Enzymatic assays
In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.
Specifications
| Antibody Conjugates |
Nickel chelate
|
|---|---|
| Application |
Protein-protein interactions
|
| Automation Compatible |
Yes
|
| Bead Type or Material |
Alpha Donor
|
| Brand |
AlphaLISA
|
| Detection Method |
Alpha
|
| Experimental Type |
In vitro
|
| Format |
Microplates
|
| One Unit Contains |
1.0 mg
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Unit Size |
1 mg
|
Video gallery
Resources
Electrophoretic Mobility Shift Assay (EMSA) is a standard technique used to study protein-DNA interactions. It is a radioactive...
Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...
Fc Gamma Receptors (FcγRs) are critical for the function of therapeutic antibodies and mediate many effector functions.
Therapeutic...
SDS, COAs, Manuals and more
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