The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Formats:
AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.
AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.
Application |
Multiplexing
|
---|---|
Assay Target Class |
Cytokine
|
Automation Compatible |
Yes
|
Brand |
AlphaPlex
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Format |
Microplates
|
One Unit Contains |
100.0 assay points
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Inflammation
|
Unit Size |
100 Assay Points
|
The AlphaPlex™ reagent system was designed to enable fast and easy transition from well-established AlphaLISA™ assays to...
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