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AlphaPlex 545 (Terbium) Human IL-1β Detection Kit, 5,000 Assay Points
AlphaPlex 545 (Terbium) Human IL-1β Detection Kit, 5,000 Assay Points
AlphaPlex™ 545 Human IL1-β Detection Kit is designed quantitative determination of human IL1-β toger with anor analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Dynamic Range | 3 - 30,000 pg/mL |
| Limit of Detection | 2.1 pg/mL |
| Limit of Quantification | 7.0 pg/mL |
| Sample Volume | 5 µL |
AlphaPlex™ 545 Human IL1-β Detection Kit is designed quantitative determination of human IL1-β toger with anor analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaPlex 545 (Terbium) Human IL-1β Detection Kit, 5,000 Assay Points
AlphaPlex 545 (Terbium) Human IL-1β Detection Kit, 5,000 Assay Points
Product information
Overview
The AlphaPlex™ 545 Human IL1ß Detection Kit is designed for the quantitative determination of human IL1ß together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
This kit contains reagents to run 5,000 assays, using a 50 µL reaction volume (5 µL of sample). Protocol can be found on the Certificate of Analysis.
AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.
AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaPlex
|
| Detection Modality |
Alpha
|
| Dynamic Range |
3 - 30,000 pg/mL
|
| Limit of Detection |
2.1 pg/mL
|
| Limit of Quantification |
7.0 pg/mL
|
| Product Group |
Kit
|
| Sample Volume |
5 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
IL1β
|
| Target Class |
Cytokines
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Inflammation
|
| Unit Size |
5,000 Assay Points
|
Video gallery
AlphaPlex 545 (Terbium) Human IL-1β Detection Kit, 5,000 Assay Points
AlphaPlex 545 (Terbium) Human IL-1β Detection Kit, 5,000 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
The AlphaPlex™ reagent system was designed to enable fast and easy transition from well-established AlphaLISA™ assays to...
Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes
This application note describes how AlphaPlex reagents from Revvity can be used to simultaneously detect IL-6 and IL-8 secretion...
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