AlphaLISA Human XIAP / BIR3 Binding Kit, 5,000 Assay Points

X-linked inhibitor of apoptosis protein (XIAP) is a member of the inhibitor of apoptosis protein (IAP) family that includes cIAP1. XIAP is a negative regulator of various apoptotic stimuli and death pathways and is the most potent caspase inhibitor of the IAP family. XIAP contains BIR domains, like other IAP family proteins, which interact with the IAP-binding motif of caspases. This interaction is implicated in the control of pro-apoptotic and anti-apoptotic activities. XIAP compound characterization on the BIR2 and BIR3 domains enables accurate profiling and selectivity studies.

XIAP is an attractive target in cancer therapies since it’s overexpression in tumor cells is a well-described mediator of resistance to chemotherapy and it has been linked to tumor aggressiveness. Several therapeutic strategies targeting XIAP have been investigated, such as SMAC mimetics. Additionally, XIAP displays E3 ligase activity and leads to targeted proteins' ubiquitination and degradation, allowing this property to be exploited via a Proteolysis-targeting chimera (PROTAC) strategy. Therefore, new compounds targeting XIAP provide dual roles, the inhibition of XIAP anti-apoptotic function and the induction of targeted protein degradation.

Formats

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 20 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 20 µL reaction volume (5 µL of sample).

Features

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.