The AlphaLISA™ immunoassay kit for human TSLP enables the quantitative determination of human TSLP in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
Features:
AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Thymic stromal lymphopoietin (TSLP) is a cytokine that plays a critical role in the maturation of T cells through antigen presenting cells. TSLP is mainly produced by epithelial cells and its expression is regulated by many other cytokine such as IL1β and TNFα. TSLP expression is linked to many disease states such as asthma, inflammatory arthritis, atopic dermatitis, eczema, eosinophilic esophagitis and other allergic states. Thus, TSLP becomes one of many important biomarkers for airway inflammatory diseases. This kit is designated to detect and quantify the levels of TSLP in cell culture media and other biological matrices.
Application |
Protein Analysis & Detection
|
---|---|
Assay Points |
100
|
Assay Target |
TSLP
|
Assay Target Class |
Biomarker
Cytokine
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Method |
Alpha
|
Format |
Microplates
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Oncology
|
Unit Size |
100 Assay Points
|
Cytokines play a vital role in both innate and adaptive immunity and are known for their ability to exert diverse functions on...
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