AlphaLISA TruHits Kit, 1,000 Assay Points

The AlphaLISA TruHits kit is designed as a tool for AlphaLISA users to identify the type of hit they have achieved during screening of compounds. The AlphaLISA TruHit kits can also be used as controls when testing the performance of an Alpha enabled instruments independently of any biology.

This kit includes AlphaLISA BSA-biotin Acceptor beads and Streptavidin Alpha Donor beads which interact together to generate an AlphaLISA signal. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. The AlphaLISA TruHits kit allows the identification of inner filters, light scatterers (insoluble compounds), singlet oxygen quenchers and biotin mimetics interfering with the AlphaLISA signal, and thus facilitates the detection of false positives.