Skip to main content
Menu
US

AlphaLISA Human TNFα Detection Kit, 500 Assay Points

AlphaLISA™ no-wash immunoassay kit detection of human TNFα in serum, buffered solution or cell culture medium.

We now offer a new and improved High Performance version of AlphaLISA Human TNFα Detection Kit, part number AL3157.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Protein Quantification
Dynamic Range 2.2 - 30,000 pg/mL
Limit of Detection 2.2 pg/mL  
Sample Volume 5 µL

AlphaLISA™ no-wash immunoassay kit detection of human TNFα in serum, buffered solution or cell culture medium.

We now offer a new and improved High Performance version of AlphaLISA Human TNFα Detection Kit, part number AL3157.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Unit Size: 500 Assay Points
Part #:
AL208C
List Price
USD 2,392.00
Unit Size: 5,000 Assay Points
Part #:
AL208F
List Price
USD 15,950.00

Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half time of an ELISA assay

AlphaLISA technology allows detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to Streptavidin-coated Donor beads while anor anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In presence of analyte, beads come into close proximity. excitation of Donor beads causes release of singlet oxygen molecules that triggers a cascade of energy transfer in Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Dynamic Range
2.2 - 30,000 pg/mL
Host Species
Human
Limit of Detection
2.2 pg/mL  
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Blue Ice
Target
TNFα
Target Class
Cytokines
Target Species
Human
Technology
Alpha
Therapeutic Area
Oncology
Unit Size
500 Assay Points

Image gallery

Video gallery

Citations

Resources

Are you looking for resources, click on the resource type to explore further.

1-5 of 5 Resources
Application Note Icon
Application Note
Automated AlphaLISA Workflows with JANUS Liquid Handlers

Immunoassays are a mainstay for the quantification of a variety of biomolecular analytes in drug discovery, drug development, and...

Brochure Icon
Brochure
Detecting cytokines with AlphaLISA guide

Cytokines are implicated in the pathology and outcome of disease states across all therapeutic areas. When preparing to run...

Flyer Icon
Flyer
Reagent solutions for autoimmunity research.

Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies

Application Note Icon
Application Note
Simultaneous detection of drug efficacy and toxicity by combining HTRF, AlphaLISA, or AlphaLISA SureFire Ultra with ATPlite

This application note demonstrates how compound’s mechanism of action and potential cytotoxic effects can be deciphered thanks to...

Flyer Icon
Flyer
Solutions to advance your CART-T cell research

Chimeric antigen receptor (CAR) T-cell therapy has transformed the field of immuno-oncology providing a novel approach to treating...

SDS, COAs, Manuals and more

Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.

Safety data sheet
+ Show more
Certificate of analysis
+ Show more
IFUs, Manuals & more
Scroll Icon