AlphaLISA™ no-wash immunoassay kit detection of human TNFα in serum, buffered solution or cell culture medium.
We now offer a new and improved High Performance version of AlphaLISA Human TNFα Detection Kit, part number AL3157.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
Features:
AlphaLISA technology allows detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to Streptavidin-coated Donor beads while anor anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In presence of analyte, beads come into close proximity. excitation of Donor beads causes release of singlet oxygen molecules that triggers a cascade of energy transfer in Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application |
Protein Quantification
|
---|---|
Assay Target |
TNFα
|
Assay Target Class |
Cytokines
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Method |
Alpha
|
Product Group |
Kit
|
Sample Volume |
5 µL
|
Species |
Human
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Oncology
|
Unit Size |
500 Assay Points
|
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