The AlphaLISA™ immunoassay kit for soluble mouse tumor necrosis factor receptor 1 (TNFR1) enables the quantitative determination of soluble mouse TNFR1 in buffered solution, serum, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
AlphaLISA features:
AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
TNFR1 is a transmembrane receptor of TNF-α and TNF-β. The soluble form (sTNFR1) is released by the cell in inflammatory response. sTNFR1 interacts with free TNF-α to inhibit inflammation. Many neurological disorders are associated with high levels of serum TNFR1 such as, schizophrenia, bipolar, and dementia. Mutations of the gene which encodes TNFR1 may lead to tumor necrosis factor receptor associated periodic syndrome (TRAPS) and/or multiple sclerosis.
Application |
Protein Analysis & Detection
|
---|---|
Assay Points |
500
|
Assay Target Class |
Biomarker
Protein
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Method |
Alpha
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Oncology
|
Unit Size |
500 Assay Points
|
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