AlphaLISA Human Periostin Detection Kit, 5,000 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Periostin, also known as osteoblast-specific factor 2, POSTN, or PN, is an extracellular matrix protein that is expressed in wide range of normal adult and fetal tissues, including lung, thyroid, periosteum, placenta, uterus, and GI tract. Periostin binds to integrins which then leads to cell migration and cell adhesion enhancement. High levels of expressed periostin in patients have been associated with cell turnover, cell division, cell invasion, and angiogenesis. It has also been associated with several inflammatory conditions. Periostin plays an important role in the development of teeth and bones but also has been shown to contribute to asthma, atopic dermatitis, and chronic rhinosinusitis.