The AlphaLISA® immunoassay kit for human matrix metalloproteinase 23 (MMP-23) enables the quantitative determination of human MMP-23 in buffer, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
AlphaLISA features:
AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Human Matrix metalloproteinase-23 (MMP23) functions as a protease. It may regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-23 is predominantly expressed in ovary, testis, and prostate indicating that it plays a specialized role in reproductive processes.
Assay Points |
500
|
---|---|
Assay Target Class |
Protein
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Method |
Alpha
|
Unit Size |
500 Assay Points
|
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