AlphaLISA Human MBNL1 Detection Kit, 5,000 assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Muscleblind-like proteins (MBNL) belong to a family of tissue-specific precursor and mature mRNA metabolism regulators in mammals. Muscleblind-like Protein 1 (MBNL1) regulates alternative splicing of pre-mRNA; it is found primarily in skeletal and cardiac muscle tissues. Increased expression of MBNL1 has been linked to myotonic dystrophy, a particular type of muscular dystrophy. The present kit permits the detection of human MBNL1 (i.e. analyte) in cell culture media and samples.