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  • Home
  • Products
  • Research & Development
  • Protein Analysis
  • Immunoassays
  • Alpha Assays
  • ALphaLISA IP-One Detection Kit, 50,000 Assay Points

ALphaLISA IP-One Detection Kit, 50,000 Assay Points

AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead image
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ALphaLISA IP-One Detection Kit, 50,000 Assay Points
AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead image
Video Play Button
AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead image
AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead
AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead image
AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead image
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The AlphaLISA™ IP-One Kit is a competitive immunoassay for quantitative detection of IP1 in cellular lysates using Alpha Technology.

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Feature Specification
Application Second Messenger Detection
Sample Volume 20 µL
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The AlphaLISA™ IP-One Kit is a competitive immunoassay for quantitative detection of IP1 in cellular lysates using Alpha Technology.

View product information
Product variants
Unit Size: 1,000 assay points
Part #:
AL3145D
List price
USD 3,341.50
Your online price:
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Unit Size: 10,000 assay points
Part #:
AL3145M
List price
USD 16,042.72
Your online price:
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Unit Size: 50,000 assay points
Part #:
AL3145R
List price
USD 50,211.07
Your online price:
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For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Total list price:
USD 50,211.07
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AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead image
AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead image
Video Play Button
ALphaLISA IP-One Detection Kit, 50,000 Assay Points
AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead image
Video Play Button
AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead image
AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead
ALphaLISA IP-One Detection Kit, 50,000 Assay Points
AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead image
AlphaLISA Competition Anti-anlyate Conjugated Acceptor Bead image
Video Play Button

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Product information

  • Overview
  • Specifications
  • Video gallery

Overview

Inositol Monophosphate (IP1) is part of the group of phosphorylated inositols that play a key role in diverse cellular functions such as cell growth, apoptosis, cell migration, endocytosis, and cell differentiation. Inositol 1,4,5 triphosphate (IP3) signals the activation of Gq coupled-receptors, however, it's short half-life makes it challenging to assess for drug screening. IP1 is a downstream metabolite of IP3, which can be made stable by the introduction of Lithium Chloride (LiCl). As such its accumulation following Gq-coupled receptor-induced phospholipase C (PLC) cascade allows for the direct characterization of all types of compounds acting on Gq-coupled receptors.

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Second Messenger Detection
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Product Group
Kit
Sample Volume
20 µL
Shipping Conditions
Shipped in Blue Ice
Target
IP1
Target Class
GPCR
Technology
Alpha
Therapeutic Area
Metabolic
Unit Size
50,000 assay points

Video gallery

ALphaLISA IP-One Detection Kit, 50,000 Assay Points
ALphaLISA IP-One Detection Kit, 50,000 Assay Points

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Emerging pathways to neuroinflammation and neurodegeneration

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