The AlphaLISA® immunoassay kit for bovine IL-18 enables the quantitative determination of bovine interleukin 18 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
Features:
AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application |
Protein Analysis & Detection
|
---|---|
Assay Points |
500
|
Assay Target Class |
Cytokine
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Format |
Microplates
|
Therapeutic Area |
Inflammation
|
Unit Size |
500 Assay Points
|
Antigen-stimulated cellular cytokine release measured by AlphaLISA bovine cytokine kits.
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