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AlphaLISA Human IL-1β Detection Kit, 500 Assay Points

AlphaLISA™ Human Interleukin-1β (IL1β) Detection Kit is designed detection and quantitation of human IL-1β in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

We now offer a new and improved High Performance version of AlphaLISA Human IL1β Detection Kit, part number AL3160.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Protein Quantification
Dynamic Range 0.65 - 30,000 pg/mL
Limit of Detection 0.65 pg/mL  
Sample Volume 5 µL

AlphaLISA™ Human Interleukin-1β (IL1β) Detection Kit is designed detection and quantitation of human IL-1β in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

We now offer a new and improved High Performance version of AlphaLISA Human IL1β Detection Kit, part number AL3160.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Unit Size: 500 Assay Points
Part #:
AL220C
List Price
USD 2,392.00
Unit Size: 5,000 Assay Points
Part #:
AL220F
List Price
USD 15,950.00

Overview

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Dynamic Range
0.65 - 30,000 pg/mL
Limit of Detection
0.65 pg/mL  
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Blue Ice
Target
IL1β
Target Class
Cytokines
Target Species
Human
Technology
Alpha
Therapeutic Area
Inflammation
Unit Size
500 Assay Points

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Resources

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Application Note
Automated AlphaLISA Workflows with JANUS Liquid Handlers

Immunoassays are a mainstay for the quantification of a variety of biomolecular analytes in drug discovery, drug development, and...

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Application Note
Rapid, no-wash measurement of immune checkpoint molecules and cytokines in co-cultures of immune cells and cancer cell lines

Breast cancer tumors can adapt to immune cell infiltration by responding to the increased concentration of interferon gamma (IFN-ɣ...

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Application Note
Simultaneous detection of drug efficacy and toxicity by combining HTRF, AlphaLISA, or AlphaLISA SureFire Ultra with ATPlite

This application note demonstrates how compound’s mechanism of action and potential cytotoxic effects can be deciphered thanks to...

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Application Note
Tracking inflammasome activity via measuring IL-1β levels with AlphaLISA and HTRF

It has been suggested that inflammation promotes malignancy via proinflammatory cytokines, such as IL-1ß, which enhance immune...

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