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AlphaLISA Human IL-1β Detection Kit, 5,000 Assay Points
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AlphaLISA Human IL-1β Detection Kit, 5,000 Assay Points
AlphaLISA™ Human Interleukin-1β (IL1β) Detection Kit is designed detection and quantitation of human IL-1β in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
We now offer a new and improved High Performance version of AlphaLISA Human IL1β Detection Kit, part number AL3160.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Dynamic Range | 0.65 - 30,000 pg/mL |
| Limit of Detection | 0.65 pg/mL |
| Sample Volume | 5 µL |
AlphaLISA™ Human Interleukin-1β (IL1β) Detection Kit is designed detection and quantitation of human IL-1β in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
We now offer a new and improved High Performance version of AlphaLISA Human IL1β Detection Kit, part number AL3160.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA Human IL-1β Detection Kit, 5,000 Assay Points
AlphaLISA Human IL-1β Detection Kit, 5,000 Assay Points
Product information
Overview
Formats:
- Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
- Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Features:
- No-wash steps, no separation steps
- ELISA alternative technology
- Sensitive detection
- Broad sample compatibility
- Small sample volume
- Results in less than 3 hours
- Half the time of an ELISA assay
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA
|
| Detection Modality |
Alpha
|
| Dynamic Range |
0.65 - 30,000 pg/mL
|
| Limit of Detection |
0.65 pg/mL
|
| Product Group |
Kit
|
| Sample Volume |
5 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
IL1β
|
| Target Class |
Cytokines
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Inflammation
|
| Unit Size |
5,000 Assay Points
|
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AlphaLISA Human IL-1β Detection Kit, 5,000 Assay Points
AlphaLISA Human IL-1β Detection Kit, 5,000 Assay Points
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AlphaLISA Human IL-1β Detection Kit, 5,000 Assay Points
AlphaLISA Human IL-1β Detection Kit, 5,000 Assay Points
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Cytokines are implicated in the pathology and outcome of disease states across all therapeutic areas. When preparing to run...
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