The AlphaLISA™ immunoassay kit for ovine interferon gamma (IFNγ) enables the quantitative determination of ovine IFNγ in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Dynamic Range | 12 - 100,000 pg/mL |
Limit of Detection | 12 pg/mL |
Limit of Quantification | 44 pg/mL |
Sample Volume | 10 µL |
The AlphaLISA™ immunoassay kit for ovine interferon gamma (IFNγ) enables the quantitative determination of ovine IFNγ in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
Features:
AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Interferon gamma becomes one of important biomarkers for determining infectious diseases in human and in domestic animals. It is critical for innate and adaptive immunity against viral, bacterial and protozoan infections. In domestic animal species, IFNγ is associated with autoinflammatory and autoimmune diseases and inhibits viral replication and bacterial infections. Ovine IFNγ has been identified as a biomarker for vaccine responses, viral, and bacterial infection. This kit is designed to detect oIFNγ in serum, plasma, and cell culture supernatants.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Modality |
Alpha
|
Dynamic Range |
12 - 100,000 pg/mL
|
Limit of Detection |
12 pg/mL
|
Limit of Quantification |
44 pg/mL
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
IFNγ
|
Target Class |
Biomarkers
|
Target Species |
Ovine
|
Technology |
Alpha
|
Therapeutic Area |
Inflammation
|
Unit Size |
100 Assay Points
|
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