AlphaLISA Human PICP (Pro-Collagen 1 C-Terminal Pro-Peptide) Detection Kit, 500 Assay Points

Collagen Type 1 is a protein present in abundance in the extracellular matrix, where forms cross-linking fibrils that associate in larger collagen fibers to play a role of structural support in connective tissues such as cartilage, bone, tendon, and skin. Due to its very unsoluble nature in its mature form, collagen is expressed and transported as a pro-collagen, in which the mature collagen sequence is flanked by two propetides respectively designated as PINP (Pro-collagen type 1 N-terminal Propeptide) and PICP (Pro-collagen type 1 C-terminal Propeptide). Mature collagen detection and quantification is not possible in immunoassays due to it being unsoluble and sticky. Instead, detection of the whole pro-collagen form or one of the propeptides is a good proxy to quantify the collagen expression in cells. In particular, targeting a propeptide like PICP allows to quantify both pro-collagen that have been expressed but not yet processed into mature collagen, and free PICP which have been cleaved from the mature sequence and are markers of collagen deposited in the extracellular matrix. The present kit is designed for the quantitative detection of Human PICP (Pro-Collagen 1 C-Terminal Pro-Peptide) in supernatants

Formats

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features

• No-wash steps, no separation steps
• ELISA alternative technology
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.