![alpha900x73.jpg](https://resources.revvity.com/images/alpha900x73.jpg?format=webp)
The AlphaLISA™ High Performance (HP) Human TNFα Detection Kit is designed for the quantitative determination of human TNFα in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
This kit is the new and improved version of part number AL208.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Tumor necrosis factor alpha (TNFα) is a multifunctional proinflammatory cytokine synthesized mainly by nucleated blood cells as a 233 aa type II transmembrane protein which is cleaved by ADAM17 between aa 76-77 to form a soluble homotrimeric complex. TNFα plays a role in lipid metabolism, coagulation, and endothelial function and has been associated with cancer, infection and inflammation (including inflammatory bowel disease), ischemia/reperfusion injury and heart failure, and insulin resistance.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application |
Protein Analysis & Detection
Protein Detection
|
---|---|
Assay Points |
5000
|
Assay Target |
TNFα
|
Assay Target Class |
Cytokine
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Shipping Conditions |
Shipped in Blue Ice
|
Target Species |
Human
|
Therapeutic Area |
Autoimmunity
Cardiovascular
Immuno-oncology
Inflammation
Neuroscience
Oncology
|
Unit Size |
5,000 Assay Points
|
Unlock the potential of whole blood analysis for a more accurate and physiologically relevant assessment of biomarkers. Whole...
Our R&D scientists were determined to enable your research by developing new and improved versions of our AlphaLISA cytokine...
Emerging pathways to neuroinflammation and neurodegeneration
Neurodegenerative diseases, such as amyotrophic lateral sclerosis...
Cytokines play an essential role in inflammatory processes, leading to their involvement in viral and bacterial infections, as...
Cytokines play a vital role in both innate and adaptive immunity and are known for their ability to exert diverse functions on...
Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies
Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.
We are here to answer your questions.