AlphaLISA Human IgG-Fc Detection Kit, 100 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well, using a 100 μL reaction volume (10 μL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 μL reaction volume (5 μL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Human Immunoglobulin G (hIgG) is one of the antibodies that is produced by plasma B-cells and protects the body from infections and toxifications such as viruses, bacteria, fungi and toxins. Human IgG is divided into 4 subtypes: IgG1, IgG2, IgG3, and IgG4. The molecular structure of human IgG is well known as a Y-like shape that contains Fab and Fc regions. The Fc portion of IgG is widely used in Fc fusion protein therapeutics for protein or antibody drug discovery and development. There are more than a dozen Fc-fusion protein drugs are available on the market to treat various human diseases. The Human IgG Fc AlphaLISA Detection Kit specifically detects human IgG Fc portion of all IgG subtypes (IgG1, IgG2, IgG3, and IgG4).

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.