AlphaLISA Human FGF23 Detection Kit, 100 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Fibroblast growth factor 23 (FGF23), a 32 kDa secreted protein, is a member of the FGF family. More specifically, it is a part of a subfamily that also includes FGF19 and FGF21. FGF23 is predominantly secreted by osteocytes and osteoblasts. The primary function is to regulate vitamin D metabolism and phosphate homeostasis. Secreted levels of FGF23 have been observed to go awry in certain tumor lines resulting in overproduction of FGF23 and hypophosphatemia. Further, elevated levels of FGF23 have been suggested as a biomarker for chronic kidney disease, where an early sign is altered phosphorus metabolism.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.