AlphaLISA Human CYR61 Detection Kit, 5,000 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

CYR61 is cysteine rich angiogenic inducer 61, also known as CCN1 is a 40-45 kDa signaling protein associated with the extracellular matrix. It regulates cellular proliferation, apoptosis, differentiation, migration, and adhesion through its interactions with different integrin receptors and through its heparin binding activity. Human CYR61 often promotes tumor growth via neovascularization, and most human tumor-derived cell lines express this protein. Therefore, CYR61 is considered a biomarker for tumor growth and for diseases related to chronic inflammation. This kit has been designed for the detection of human CYR61 in serum and cell culture media.