AlphaLISA Human Cereblon Binding Kit, 5,000 Assay Points

Cereblon (CRBN) is a ubiquitously expressed protein which functions as a substrate recognition subunit within CRL4-CRBN E3 ubiquitin ligase complex and targets specific proteins proteolysis through ubiquitin proteasome system (UPS). Cereblon plays a key role in normal limb outgrowth, normal cell proliferation and metabolism, and in memory and learning. Cereblon is also primary direct target of immunomodulatory drugs Thalidomide, Pomalidomide, and Lenalidomide, which are FDA approved treating multiple myeloma. Like or E3 ligases such as VHL, Cereblon E3 ligase property is commonly used in targeted protein degradation strategy, eg PROTAC.

Formats:

• Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 384-well format, using a 20 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume

AlphaLISA technology allows detection of molecules of interest in a no- wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to Streptavidin-coated Donor beads while anor anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In presence of analyte, beads come into close proximity. excitation of Donor beads causes release of singlet oxygen molecules that triggers a cascade of energy transfer in Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

This product is also available using HTRF Technology.