The AlphaLISA™ immunoassay kit for AAV9 enables the quantitative determination of AAV9 capsids in buffer, cell culture media, cell lysates and cell supernatants using a homogeneous AlphaLISA assay (no wash steps).
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Adeno-associated virus (AA) vectors are the leading platform for gene delivery for the treatment of a variety of human diseases. AAV Serotype 9 used as a vector in gene therapy has been particularly developed for the treatment of neurological diseases mainly because of its ability to cross the blood-brain barrier to target the central nervous system (astrocytes and neurons). It also exhibits a tropism for other tissues such as liver, muscle, or lung. The AAV9 kit is designed to detect and quantify AAV9 particles.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is captured by AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Application |
Gene Therapy
|
---|---|
Assay Points |
100
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
100 Assay Points
|
Reliable, Quantitative, and No-Wash Assay Platform for AAV Capsid Detection
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