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AlphaLISA Lysis Buffer Selection Kit
AlphaLISA Lysis Buffer Selection Kit
AlphaLISA Target Engagement (TE) Buffers can be used to prepare cellular lysates for cell-based target engagement assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA Target Engagement (TE) Buffers can be used to prepare cellular lysates for cell-based target engagement assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA Lysis Buffer Selection Kit
AlphaLISA Lysis Buffer Selection Kit
Product information
Overview
This kit contains 10 mL each of buffers 1, 2, 3, 4, and 5 for use in cell-based target engagement assays.
AlphaLISA TE Cell Lysis Buffer 1: This formulation is the most aggressive detergent formulation, containing divalent cation chelators, low salt concentration, moderate glycerol concentration.
AlphaLISA TE Cell Lysis Buffer 2: Less aggressive detergent formulation, optimized for lysis of a broad range of cells without releasing nuclear DNA and minimally disrupting protein interactions; physiological salt concentrations.
AlphaLISA TE Cell Lysis Buffer 3: Medium detergent concentration, strongest pH buffering capacity, no glycerol, low salt, 0.1% casein
AlphaLISA TE Cell Lysis Buffer 4: Medium-High detergent concentration, contains divalent cation chelators, close to physiological osmotic strength, no glycerol
AlphaLISA TE Cell Lysis Buffer 5: Medium-High detergent concentration, contains divalent cation chelator, close to physiological osmotic strength, highest glycerol concentration
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA
|
| Buffer/Solvent |
Buffers 1, 2, 3, 4, and 5
|
| Detection Modality |
Alpha
|
| Product Group |
Buffer
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target Class |
Phosphoproteins
|
| Technology |
Alpha
|
| Unit Size |
50 mL
|
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