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AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, 500 Assay Points
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AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, 500 Assay Points
The AlphaLISA™ SureFire® Ultra™p-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™p-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, 500 Assay Points
Product information
Overview
The AlphaLISA™ SureFire® Ultra™ p-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGF Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
In the AlphaLISA™ SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA™ SureFire® Ultra™ kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire® kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Cellular or Signaling Pathway |
Receptor signaling
|
| Cellular Process |
Receptor signaling
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
VEGF2 Receptor
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Unit Size |
500 Assay Points
|
Image gallery
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, 500 Assay Points
Video gallery
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, 500 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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