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AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA SureFire Ultra phospho-VEGFR2 (Tyr1175) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-VEGFR2 assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
Extra control lysate for the AlphaLISA SureFire Ultra phospho-VEGFR2 (Tyr1175) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-VEGFR2 assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-VEGF Receptor 2 (Tyr1175) assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. This control lysate was prepared from HUVEC cells grown to confluence in T175 flasks, and then serum-starved overnight. The following day the media was removed, and the cells were treated with 100 ng/mL VEGF for 10 minutes. The agonist was removed, and the cells were lysed with 4 mL of freshly prepared 1X Lysis buffer, with shaking for 10 minutes.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
VEGFR2
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Unit Size |
1 Vial
|
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AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Phospho-VEGF Receptor 2 (VEGFR2) (Tyr1175) Detection Kit, Control Lysate
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