The AlphaLISA™ SureFire® Ultra™ Human Phospho-Ubiquitin (Ser65) assay is a sandwich immunoassay for quantitative detection of phospho-ubiquitin (Ser65) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Ubiquitin is a highly conserved regulatory protein found in most tissues of eukaryotic organism. Phosphorylation of ubiquitin is a key step in the process of mitochondria quality control through the Parkin/PINK-1 interaction. Parkin is a ubiquitin ligase E3 that conjugates ubiquitin to impaired-mitochondrial proteins, leading to organelle degradation. PINK1 is a Ser/Thr kinase that accumulates only on impaired mitochondria and phosphorylates two substrates, the ubiquitin-like domain of Parkin and ubiquitin chains. The amplified Parkin/PINK-1 dependent ubiquitin functions act as a signal for mitophagy. Dysregulation of this signaling is linked to neurodegenerative diseases such as Parkinson's Disease.
The AlphaLISA SureFire Ultra Human Phospho-Ubiquitin (Ser65) assay is a sandwich immunoassay for the quantitative detection of phospho-Ubiquitin (Ser65) in cellular lysates, using Alpha Technology.
Application |
Protein Detection
|
---|---|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Shipping Conditions |
Shipped in Blue Ice
|
Target Species |
Human
|
Therapeutic Area |
Central Nervous System
|
Unit Size |
10,000 Assay Points
|
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
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