Tyrosine kinase 2 (TYK2) is a member of the JAK1, JAK2, and JAK3 family of non-receptor Janus tyrosine kinases. Phosphorylation of TYK2 is induced by a broad range of cytokines and growth factors (IL12, IL23, and interferons) bound to their receptors. The main substrates, STATs, and cytokine receptors are among the other targets that the activated TYK2 goes on to phosphorylate. Cell division, migration, apoptosis, and proliferation are all accelerated by JAKs/STATs signaling. An appealing aim for anti-inflammatory treatments is to modify JAK/STATs signaling to lessen cytokine-induced pro-inflammatory responses.

The AlphaLISA SureFire Ultra Human Total TYK2 Detection Kit is a sandwich immunoassay for the quantitative detection of total TYK2 in cellular lysates, using Alpha Technology.

 Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening