The TEK receptor tyrosine kinase (TIE2), encoded by the TEK gene in humans, is primarily expressed on endothelial cells. Upon binding with angiopoietin-1 (Angpt1), TIE2 induces angiogenesis and supports endothelial cell survival. This activation leads to downstream signaling pathways, including MEK/ERK, IKKb/NFkB, and AKT. Consequently, TIE2 contributes to vascular stability, cellular proliferation, inflammation, and cell survival. Elevated levels of Ang1 and/or Ang2 have been observed in patients with various cancers.

The AlphaLISA SureFire Ultra Human Phospho-TIE2 (Tyr992) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-TIE2 in cellular lysates, using Alpha technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput drug screening