Transcription factor EB (TFEB) plays a key role in the regulation of basic cellular processes, including lysosomal biogenesis and autophagy. While phosphorylated TFEB is found in the cytoplasm, dephosphorylated TFEB moves to the nucleus to initiate the transcription of target genes. The subcellular location and function of TFEB are regulated by phosphorylation at the lysosomal surface, which is controlled by mechanistic target of rapamycin (mTOR). TFEB is implicated in various diseases, such as lysosomal storage disorders, neurodegenerative diseases, cancers, and metabolic disorders.

The AlphaLISA SureFire Ultra Mouse Phospho-TFEB (Ser122) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-TFEB (Ser122) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening