The AlphaLISA® SureFire® Ultra™ phospho-Syk assay is a sandwich immunoassay for quantitative detection of Syk (phosphorylated on Tyr352) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA SureFire Ultra™ kits are compatible with:
Alpha SureFire kits can be used for:
Application |
Kinase
Protein Analysis & Detection
|
---|---|
Assay Points |
500
|
Assay Target Class |
Phospho-protein
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Unit Size |
500 Assay Points
|
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