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AlphaLISA SureFire Ultra Human Phospho-STAT4 (Tyr693) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-STAT4 (Tyr693) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-STAT4 (Tyr693) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Cell Signaling

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-STAT4 (Tyr693) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-STAT4 (Tyr693) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variant
Unit Size: 1 Vial
Part #:
ALSU-PST4-A-L
List Price
USD 142.66

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-STAT4 (Tyr693) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. The lysate is prepared from NK-92 cells, cultured to 0.2 x 106 cells/mL in T175 flasks in 12.5% FBS containing medium, IL-2 starved, then treated with 200 ng/mL IL-12 for 30 minutes and lysed at 3.2 x 106 cells/mL with 1X SureFire Ultra Lysis Buffer.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Control Lysate
Shipping Conditions
Shipped in Blue Ice
Target
STAT4
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Unit Size
1 Vial

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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