AlphaLISA SureFire Ultra Human & Mouse Phospho-STAT3 (Tyr705 ) Detection Kit, 100 Assay Points

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA SureFire Ultra p-STAT3 (Tyr705) assay, donor beads are coated with streptavidin to capture one of the antibodies used in the assay, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, leading to signal generation. The intensity of the light emission is directly proportional to the amount of phosphoprotein present in the sample, making the AlphaLISA SureFire Ultra an excellent immunoassay for quantitative detection of phospho-STAT3 in cellular lysates.

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) kits are compatible with cell and tissue lysates, antibody modulators, and biotherapeutic antibodies. These kits can be used for cellular kinase assays, receptor activation studies, and screening.