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AlphaLISA SureFire Ultra Human & Mouse Phospho-STAT1 (Ser727) Detection Kit, 500 Assay Points
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AlphaLISA SureFire Ultra Human & Mouse Phospho-STAT1 (Ser727) Detection Kit, 500 Assay Points
The AlphaLISA™ SureFire® Ultra™ p-STAT1 (Ser727) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Ser727) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ p-STAT1 (Ser727) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Ser727) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human & Mouse Phospho-STAT1 (Ser727) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Phospho-STAT1 (Ser727) Detection Kit, 500 Assay Points
Product information
Overview
The AlphaLISA™ SureFire® Ultra™ p-STAT1 (Ser727) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Ser727) in cellular lysates using Alpha Technology.
This kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
In the AlphaLISA™ SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA™ SureFire® Ultra™ kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire® kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Cellular or Signaling Pathway |
JAK/STAT
|
| Cellular Process |
JAK/STAT
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
STAT1
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Unit Size |
500 Assay Points
|
Image gallery
AlphaLISA SureFire Ultra Human & Mouse Phospho-STAT1 (Ser727) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Phospho-STAT1 (Ser727) Detection Kit, 500 Assay Points
Video gallery
AlphaLISA SureFire Ultra Human & Mouse Phospho-STAT1 (Ser727) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Phospho-STAT1 (Ser727) Detection Kit, 500 Assay Points
Resources
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Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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