Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
AlphaLISA SureFire Ultra Human & Mouse Total SMAD4 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total SMAD4 Detection Kit, 500 Assay Points
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total SMAD4 assay is a sandwich immunoassay for quantitative detection of total SMAD4 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total SMAD4 assay is a sandwich immunoassay for quantitative detection of total SMAD4 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human & Mouse Total SMAD4 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total SMAD4 Detection Kit, 500 Assay Points
Product information
Overview
SMAD family member 4 (SMAD4), also known as mothers against decapentaplegic homolog 4, or DPC4 (deleted in pancreatic cancer-4), belongs to the SMAD family of transcription factor proteins, which act as mediators of TGF-β signal transduction. In conjunction with SMAD 2 and SMAD 3, the SMAD2/3/4 complex forms and acts as a transcription factor that promotes the expression of a pro-fibrotic phenotype. Mutated SMAD4 is found in many types of cancers and fibrosis.
The AlphaLISA SureFire Ultra Human and Mouse Total SMAD4 Detection Kit is a sandwich immunoassay for the quantitative detection of total SMAD4 in cellular lysates, using Alpha Technology.
Formats
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
SMAD4
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Therapeutic Area |
Metabolic
Oncology
|
| Unit Size |
500 Assay Points
|
Video gallery
AlphaLISA SureFire Ultra Human & Mouse Total SMAD4 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total SMAD4 Detection Kit, 500 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
How can we help you?
We are here to answer your questions.
