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AlphaLISA SureFire Ultra Human & Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra phospho-SMAD2 assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-SMAD2 assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Offer EXTENDED - Save 10% on this product until September 23rd.

Promo code: SAVE2410
Part number: ALSU-PSM2-A-L
List price: USD 142.66
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USD 142.66
USD 142.66 /each

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-SMAD2 assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, RAW 264.7 cells were cultured to confluence in T175 flasks in 10% FBS-containing medium, then treated with 10 ng/mL rhTGFβ for 90min and lysed in 6 mL of 1X SureFire Ultra Lysis buffer.

Specifications

Application
Kinase
Protein Analysis & Detection
Assay Pathway
TGFß/SMAD
Assay Target Class
Phospho-protein
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Metabolic
Unit Size
Vial

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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