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AlphaLISA Surefire Ultra Human and Mouse Phospho-SMAD2 (Ser465/467), Control Lysate
Extra control lysate for the AlphaLISA SureFire Ultra phospho-SMAD2 assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-SMAD2 assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-SMAD2 assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, RAW 264.7 cells were cultured to confluence in T175 flasks in 10% FBS-containing medium, then treated with 10 ng/mL rhTGFβ for 90min and lysed in 6 mL of 1X SureFire Ultra Lysis buffer.
Specifications
| Application |
Kinase
Protein Analysis & Detection
|
|---|---|
| Assay Pathway |
TGFß/SMAD
|
| Assay Target Class |
Phospho-protein
|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Method |
Alpha
|
| Therapeutic Area |
Metabolic
|
| Unit Size |
Vial
|
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Resources
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
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