Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-SLP76 (Tyr113) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-SLP76 (Tyr113) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-SLP76 (Tyr113) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-SLP76 (Tyr113) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-SLP76 (Tyr113) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate Jurkat cells were cultured in T175 flask to 106 cells/mL in 10% FBS containing medium, then treated with 11 mM H2O2 and 50 nM Calyculin A for 10 minutes and lysed at 3.2X106 cells/mL with 1X SureFire Ultra Lysis Buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
SLP76
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Unit Size |
1 Vial
|
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