Feature | Specification |
---|---|
Application | Cell Signaling |
Protocol Time | 2h at RT |
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Total SIRPα Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were cultured in 10% FBS containing medium. Cells were harvested, washed with HBSS, adjusted to 106 cells/mL and treated with 500 μM Pervanadate for 15 minutes. Cells were lysed with the addition of 5X Lysis Buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Protocol Time |
2h at RT
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
SIRPα
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Therapeutic Area |
Autoimmunity
Oncology
|
Unit Size |
1 vial
|
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