The AlphaLISA™ SureFire® Ultra™ Total RSK1 assay is a sandwich immunoassay for quantitative detection of total RSK1 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two non-identical, functional kinase domains and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs). Several sites, both within and outside the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are essential for kinase activation. RSK1-3 is activated via a coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters.
The AlphaLISA™ SureFire® Ultra™ Total RSK1 assay is a sandwich immunoassay for quantitative detection of total RSK1 in cellular lysates using Alpha Technology.
Formats:
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
Application |
Protein Analysis & Detection
Protein Detection
|
---|---|
Assay Target |
Rsk1
|
Assay Target Class |
Phospho-protein
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Oncology
|
Unit Size |
50,000 Assay Points
|
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
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