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AlphaLISA SureFire Ultra Human Phospho-RSK1 (Thr573) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-RSK1 (Thr573) Detection Kit, 500 Assay Points
The AlphaLISA™ SureFire® Ultra™ Phospho-RSK1 (Tyr573) assay is a sandwich immunoassay for quantitative detection of phospho RSK1 (Tyr573) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Phospho-RSK1 (Tyr573) assay is a sandwich immunoassay for quantitative detection of phospho RSK1 (Tyr573) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Phospho-RSK1 (Thr573) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-RSK1 (Thr573) Detection Kit, 500 Assay Points
Product information
Overview
Serine/threonine-protein kinase acts downstream from ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81, and NR4A1/NUR77. It regulates translation through RPS6 and EIF4B phosphorylation and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing the pro-apoptotic function of BAD and DAPK1.
Upon extracellular signal or mitogen stimulation, RSK1 is phosphorylated at Thr-573 in the C-terminal kinase domain (CTD) by MAPK1/ERK2 and MAPK3/ERK1.
The AlphaLISA™ SureFire® Ultra™ Phospho-RSK1 (Tyr573) assay is a sandwich immunoassay for quantitative detection of phospho-RSK1 in cellular lysates using Alpha Technology.
Formats:
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
Rsk1
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Oncology
|
| Unit Size |
500 Assay Points
|
Video gallery
AlphaLISA SureFire Ultra Human Phospho-RSK1 (Thr573) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-RSK1 (Thr573) Detection Kit, 500 Assay Points
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