AlphaLISA SureFire Ultra Human & Mouse Total RBM39 Detection Kit, 500 Assay Points

RNA binding motif protein 39 (RBM39), also known as CAPERα, HCC1, and RNPC2, is an RNA binding protein (RBP) and splicing factor that plays a role in maintaining transcript variant homeostasis. RBM39 promotes exon inclusion via regulation of exon cassette splicing and is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha, ESR2/ER-beta, and JUN/AP-1, independently of the pre-mRNA splicing factor activity. RBM39 has been deemed essential for cancer cell survival and is associated with acute myeloid leukemia (AML), myeloma malignancy, breast cancer, and lung cancer.

The AlphaLISA SureFire Human and Mouse Total RBM39 Detection Kit is a sandwich immunoassay for the quantitative detection of total RBM39 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening