Rab29, also known as Ras-related protein Rab-29, Rab-7-like protein 1, and Ras-related protein Rab-29, is encoded by the RAB29 gene. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that can recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB29 and LRRK2 play a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Rab29 recruits LRRK2 to the Golgi complex and stimulates LRRK2 kinase activity. Rab29 is a target for the treatment of neurodegenerative diseases and intracellular membrane trafficking-related diseases.

The AlphaLISA SureFire Ultra Human Total RAB29 Detection Kit is a sandwich immunoassay for the quantitative detection of total RAB29 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening