Phosphatase and tensin homolog, also known as PTEN, is a 402 AA protein that is expressed throughout the body. PTEN primarily functions by dephosphorylating phosphatidylinositol 3,4,5-triphosphate, which inhibits the AKT signaling pathway, and is essential for cell survival. It was discovered via this interaction that PTEN is essential for the inhibition of tumors. PTEN expression is frequently absent or drastically decreased in malignancies of the breast, ovary, endometrial, prostate, and lung.

The AlphaLISA SureFire Ultra Human and Mouse Total PTEN Detection Kit is a sandwich immunoassay for the quantitative detection of total PTEN in cellular lysates, using Alpha Technology.

 Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening