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AlphaLISA Surefire Ultra Human Phospho-Rb (Thr821/826), Control Lysate
Extra control lysate for the AlphaLISA® SureFire® Ultra™ phospho-Rb (Thr821/826) assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-Rb assay kit but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
This positive control lysate is prepared from Jurkat cells cultured in a T175 flask to 10 6 cells/mL in medium containing 10%FBS, treated with 2.5mM Pervanadate for 30 minutes, and lysed at 3.2x106 cells/mL with 1X SureFire Ultra Lysis buffer.
Specifications
| Application |
Protein Analysis & Detection
|
|---|---|
| Assay Target Class |
Phospho-protein
|
| Assay Technology |
Alpha
|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Method |
Alpha
|
| Experimental Type |
In vitro
|
| Therapeutic Area |
Oncology
|
| Unit Size |
1 Vial
|
Video gallery
Resources
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
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