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AlphaLISA SureFire Ultra Human & Mouse Total PLK1 Detection Kit, 10,000 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total PLK1 Detection Kit, 10,000 Assay Points
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total PLK1 assay is a sandwich immunoassay for quantitative detection of total PLK1 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total PLK1 assay is a sandwich immunoassay for quantitative detection of total PLK1 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human & Mouse Total PLK1 Detection Kit, 10,000 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total PLK1 Detection Kit, 10,000 Assay Points
Product information
Overview
Polo-like kinase 1 (PLK1) is a Ser/Thr kinase essential for cell division (mitosis) and as such, a key regulator of the G2/M checkpoint of the cell cycle. PLK1 is activated by phosphorylation of Thr210 by AurA kinase and active PLK1 phosphorylates CDC25. Overexpression of PLK1 is associated with many types of cancers, including lung, breast, liver, and osteosarcoma.
The AlphaLISA SureFire Ultra Human and Mouse Total PLK1 is a sandwich immunoassay for the quantitative detection of total PLK1 in cellular lysates, using Alpha Technology.
Formats
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
PLK1
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Therapeutic Area |
Neuroscience
Oncology
|
| Unit Size |
10,000 Assay Points
|
Video gallery
AlphaLISA SureFire Ultra Human & Mouse Total PLK1 Detection Kit, 10,000 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total PLK1 Detection Kit, 10,000 Assay Points
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Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
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