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AlphaLISA SureFire Ultra Human Phospho-PLC-γ2 (Tyr1217) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr1217) assay. This control is already provided in the AlphaLISA SureFire UltraPhospho-PLC G2 (Tyr1217) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Cell Signaling

Extra control lysate for the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr1217) assay. This control is already provided in the AlphaLISA SureFire UltraPhospho-PLC G2 (Tyr1217) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variant
Unit Size: 1 Vial
Part #:
ALSU-PPLCG2-D-L
List Price
USD 29.37

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr1217), or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP1 cells were cultured in T175 flasks to between 0.6-0.8 x 106 cells/mL in 10% FBS containing medium. Cells were washed, then treated with 0.25 mM Pervanadate in HBSS for 30 minutes and lysed to 4 x 106cells/mL with 5X SureFire Ultra Lysis buffer.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Host Species
Human
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Control Lysate
Shipping Conditions
Shipped in Blue Ice
Target
PLC G2
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Autoimmunity
Central Nervous System
Inflammation
Oncology
Unit Size
1 Vial

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