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AlphaLISA Surefire Ultra Human Phospho-PLC G2 (Tyr1217), Control Lysate
Extra control lysate for the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr1217) assay. This control is already provided in the AlphaLISA SureFire UltraPhospho-PLC G2 (Tyr1217) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr1217), or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP1 cells were cultured in T175 flasks to between 0.6-0.8 x 106 cells/mL in 10% FBS containing medium. Cells were washed, then treated with 0.25 mM Pervanadate in HBSS for 30 minutes and lysed to 4 x 106cells/mL with 5X SureFire Ultra Lysis buffer.
Specifications
| Application |
Protein Detection
|
|---|---|
| Assay Technology |
Alpha
|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Experimental Type |
In vitro
|
| Species |
Human
|
| Therapeutic Area |
Autoimmunity
Central Nervous System
Inflammation
Oncology
|
| Unit Size |
1 Vial
|
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