Extra control lysate for the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr1217) assay. This control is already provided in the AlphaLISA SureFire UltraPhospho-PLC G2 (Tyr1217) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Cell Signaling |
Extra control lysate for the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr1217) assay. This control is already provided in the AlphaLISA SureFire UltraPhospho-PLC G2 (Tyr1217) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-PLC G2 (Tyr1217), or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP1 cells were cultured in T175 flasks to between 0.6-0.8 x 106 cells/mL in 10% FBS containing medium. Cells were washed, then treated with 0.25 mM Pervanadate in HBSS for 30 minutes and lysed to 4 x 106cells/mL with 5X SureFire Ultra Lysis buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Host Species |
Human
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
PLC G2
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Therapeutic Area |
Autoimmunity
Central Nervous System
Inflammation
Oncology
|
Unit Size |
1 Vial
|
Are you looking for resources, click on the resource type to explore further.
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
We are here to answer your questions.