Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
AlphaLISA SureFire Ultra Human Total Parkin Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Total Parkin Detection Kit, 50,000 Assay Points
The AlphaLISA™ SureFire® Ultra™ Human Total Parkin assay is a sandwich immunoassay for quantitative detection of total Parkin in cellular lysates using Alpha Technology.
For research use only. Not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human Total Parkin assay is a sandwich immunoassay for quantitative detection of total Parkin in cellular lysates using Alpha Technology.
For research use only. Not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Total Parkin Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Total Parkin Detection Kit, 50,000 Assay Points
Product information
Overview
Parkin, encoded by the PARK2 gene, is an E3 ubiquitin ligase which has a neuroprotective role in combination with the Ser/Thr kinase PINK1 by acting in a mitochondrial quality control pathway. Since mitochondria are continuously exposed to intra and extra mitochondrial threats including reactive oxygen species (ROS), the removal of damaged mitochondria is critical for maintaining cellular homeostasis. Dysfunction of Parkin represents both a predominant cause of familial parkinsonism but also a formal risk factor for the more common, sporadic form of Parkinson's disease (PD).
The AlphaLISA SureFire Human Total Parkin Detection Kit is a sandwich immunoassay for the quantitative detection of total Parkin in cellular lysates, using Alpha Technology.
Formats:
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
Parkin
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Neuroscience
|
| Unit Size |
50,000 Assay Points
|
Video gallery
AlphaLISA SureFire Ultra Human Total Parkin Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Total Parkin Detection Kit, 50,000 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
How can we help you?
We are here to answer your questions.
