AlphaLISA SureFire Ultra Human Phospho-Parkin (Ser65) Detection Kit, 500 Assay Points

Parkin, encoded by the PARK2 gene, is an E3 ubiquitin ligase which has a neuroprotective role in combination with the Ser/Thr kinase PINK1 by acting in a mitochondrial quality control pathway. Since mitochondria are continuously exposed to intra and extra mitochondrial threats including reactive oxygen species (ROS), the removal of damaged mitochondria is critical for maintaining cellular homeostasis. Dysfunction of Parkin represents both a predominant cause of familial parkinsonism but also a formal risk factor for the more common, sporadic form of Parkinson's disease (PD).

The AlphaLISA SureFire Human Phospho-Parkin (Ser65) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Parkin (Ser65) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening